The scientific method is a process for experimentation that is used to explore observations and answer questions. Scientists use the scientific method to search for cause and effect relationships in nature. In other words, they design an experiment so that changes to one item cause something else to vary in a predictable way. Each of these steps will help to insure that your experiment is sound. The first step is to observe something and question it. Questions such as how, what, if, when, or why are a good starting point. The second step is to try to research and see if any other scientists have answered this question and what their hypothesis was and if they made any mistakes in their experiment. Finally one can form a hypothesis of their own. You must state your hypothesis in a way that you can easily measure, and of course, your hypothesis should be constructed in a way to help you answer your original question. Next, one must test the hypothesis by doing an experiment. Your experiment tests whether your hypothesis is supported or not. It is important for your experiment to be a fair test. You conduct a fair test by making sure that you change only one factor at a time while keeping all other conditions the same. You should also repeat your experiments several times to make sure that the first results weren’t just an accident. Once your experiment is complete, you collect your measurements and analyze them to see if they support your hypothesis or not. The final step is to share your hypothesis and data with others.
The Biology 102/103 Lab 4 Experiment 1: Effect of Enzyme Concentration and Experiment 2: Effect of Temperature on Enzyme Activity assignments are relatively simple for many students but require some prior knowledge.
In Experiment 1: Effect of Enzyme Concentration, students will be required to conduct an experiment to determine how the concentration of enzymes affects the overall production of gas by measuring the volume of gas produced in a balloon. Students will be required to measure the circumference of the balloon after one minute, three minutes and five minutes and record their findings. Then, they will need to answer a series of questions regarding their findings.
In Experiment 2: Effect of Temperature on Enzyme Activity, students will conduct the same experiment with different parameters. They will need to measure the production of gas in the following temperature settings:
· In a refrigerator;
· At room temperature;
· In hot water at 85 degrees Celsius; and
· In boiling water at 100 degrees Celsius.
Like the previous experiment, measurements will be taken after one minute, three minutes and five minutes. Finally, a series of questions regarding the students’ findings will be answered.
Of all of the Biology 102/103 labs, Experiment 1: Effect of Enzyme Concentration and Experiment 2: Effect of Temperature on Enzyme Activity assignments are usually the most easily understood. In each case, you will be given balloons containing a gas-producing enzyme. After activating the enzyme inside the balloon, you will need to record the production of gas by measuring the circumference of the balloon.
For Experiment 1: Effect of Enzyme Concentration, you will need to identify both the enzyme and the substrate being used. You will also need to identify how the amount of enzyme affects the production of gas. You will also need to draw a graph illustrating how the gas production would be affected if the substrate was increased or decreased. Finally, you will need to identify a way in which the compound known as hydrogen peroxide can be changed to become non-toxic inside the human body. For this, you should take a look at the chemical formula. Hydrogen peroxide’s chemical formula is H202, and it can be easily changed to pure H20 – or water – with the addition of the enzyme catalase.
For Experiment 2: Effect of Temperature on Enzyme Activity, you will need to take your experiment a bit further. Again, you will need to measure the amount of gas produced by certain enzymes, but you will need to do so under various conditions. These conditions are all related to temperature, and you will need to not only record your findings, but also explain how these different temperatures affected the production of gas. Some of the questions you will need to answer include identifying the enzyme and substrate and then explaining how the enzyme activity can be increased in conditions where it is low. You will also need to design your own experiment to determine the best temperature for maximum enzyme activity complete with control samples.
The BIO 103 LAB 9 Final assessment has proven to be a difficult task for many students. While this lab appears to be difficult it is in fact much simpler than one might expect. Here are the directions for the lab:
Refer to your DRAFT Hypothesis and Design. Review the “Scientific Method Tutorial” (all six parts) found in “Science Learning Center” in “Course Resources” in the “Course Content” area of our electronic LEO classroom. Did you apply these concepts to your process? Some questions to consider are below, others can be found in your review of the “Scientific Method Tutorial.” The main goal for this task is for you to recast your DRAFT into a final high quality document. The questions below are meant to help you identify items that may need further revision.
Is your hypothesis testable? Can it provide predictions that are testable? Can it potentially be shown to be false by further observations or experimentation? Was your experiment designed to disprove your hypothesis?
What are the variables in your experiment? How many variables are there? Have you controlled for all of these influences?
Are there practical limits for the experiment? Are there variables for which you cannot control?
If you did this experiment, what do you think the results would be? Why do you think this?
In your conclusions, did you avoid using language such as “the experiment proved the hypothesis.”
Conduct informational research on the topic. Summarize the results of this literature search and include references.
Can you find experiments that have been conducted that have an experimental design similar to yours?
If possible, conduct the experiment or some part of the experiment. Collect preliminary data. (Optional, Not Required)
The lab 9 assessment should be thought of as an experiment blueprint. The best way to approach this lab is to create a simple lab idea (perhaps one that has already been performed but researchers). You should research biology experiments for this assessment and try to find ones that are of importance to you. For example, if you decide to perform an experiment concerning protein absorption, you might want to relate this to weight loss. It should also be noted that this lab is usually written in a report or research paper form but on occasion it is done like other labs where they are simply question and answer. If you need additional assistance contact me at Homework Mountain. Good luck!